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©CECT, University of Valencia

Temporal temperature gradient gel electrophoresis (TTGE)

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DNA amplification by PCR of a conserved molecular marker followed by separation of the fragments in a denaturing acrylamide gel electrophoresis under a gradient of temperature. Used to study the microbial diversity of an environmental sample

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Service Category

Molecular typing and phylogenetic analysis

Service Sub-Category

Genotyping

Service Sub-Category Description

Strain typing based on differences in the DNA sequence of the isolates or environmental samples. Used, for example, for strain typing/differentiation, for phylogenetic analysis or to study the microbial diversity of environmental samples. The service includes consultancy to define the most suitable method considering the type of sample or the taxonomic group of the strain of interest

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